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Chinese Journal of Preventive Medicine ; (12): 499-501, 2008.
Article in Chinese | WPRIM | ID: wpr-352456

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the contamination of Staphylococcus aureus isolates in ice cream by phenotypic typing and molecular typing.</p><p><b>METHODS</b>The Staphylococcus aureus isolates were separated from ice cream, filler, cutter, salves and material. The separated isolates were characterized by drug-resistance, staphylococcal enterotoxin (SEA-E), SE (A-E, G-J) genes and pulsed-field gel electrophoresis (PFGE) types.</p><p><b>RESULT</b>Two Staphylococcus aureus isolates were separated, one from ice cream, another from cutter. Their characteristics of drug-resistance, staphylococcal enterotoxin (SEA-E), SE (A-E,G-J) genes and PFGE type were the same.</p><p><b>CONCLUSION</b>The two Staphylococcus aureus isolates were the same clone. The contaminated Staphylococcus aureus isolates could be traced to the contaminated cutters.</p>


Subject(s)
Anti-Bacterial Agents , Pharmacology , Bacterial Typing Techniques , Electrophoresis, Gel, Pulsed-Field , Enterotoxins , Genetics , Food Microbiology , Ice Cream , Microbiology , Microbial Sensitivity Tests , Staphylococcus aureus , Classification
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